Fig. 7.

Testing inhibitors of the self-palmitoylation reaction. a Palmitate or 2-bromo-palmitate at the indicated final concentrations were incubated with wild-type GST-Bet3 and [³H]-Pal-CoA for 15 min at 30°C. b Lyso-phosphatidylcholine, cerulenin or tunicamycin at the indicated final concentrations were incubated with wild-type GST-Bet3 and [³H]-Pal-CoA for 15 min at 30°C. #: 1% chloroform, the solvent for the three inhibitors, was added to the sample. c The designated nucleotides at the indicated final concentrations and MgCl₂ (1 mM) were incubated with wild-type GST-Bet3 and [³H]-Pal-CoA for 15 min at 30°C