Fig. 5.

Evaluation of neuroprotective properties of DDC, aphidicolin and 1,5-isoquinonediol on MPP⁺-induced neurotoxicity in CGC. The bar chart shows the effects of the three different (compounds) tested on 200 μM MPP⁺-induced toxicity in CGNs exposed for 24 h. Viability is based on MTT assays, apoptotic cells are based on flow cytometry analyses and condensed nuclei counting by propidium iodide staining under fluorescence illumination. The nuclei were counted on a fluorescence microscope, distinguishing normal nuclei from the condensed ones following the criteria stated in “Materials and methods”. Each point is the mean ± SEM of four wells of five to six different cultures. Statistical significance was determined by one-way ANOVA followed by Tukey’s tests: **p < 0.01 and ***p < 0.001 versus control