Fig. 6.

aBar chart showing the quantitation of the percentage of BdrU incorporation into DNA induced by MPP⁺ and 24 h S/K deprivation (positive control) on CGC in the absence or presence of various concentrations of Iso (25 μM), Aphi (30 μM), DCC 30 mM. Each point is the mean ± SEM of three to four cultures, carried out in duplicate. The statistical analysis used was non-parametric ANOVA followed by Tukey’s test: *p < 0.05 and ***p < 0.001 versus MPP⁺ and S/K deprivation and ^#p < 0.05; ^##p < 0.01 versus treatment Iso and DCC and ^&&&p < 0.01 versus treatment Aphi and DCC. b Western-blot analysis of the levels of cyclin A, DNA polymerase β, and DNA polymerase δ in CGC after treatment with MPP⁺ 200 μM. Cultures were treated with MPP⁺ 200 μM for different times. At the end of the treatments, cells were lysed and the cell lysates were subjected to immunoblot analysis with an antibody directed against cyclin A, DNA polymerase β and DNA polymerase δ (see “Materials and methods”). c Western-blot analysis of cycling A and DNA polymerase β in B65 cells. Changes in the band intensities were calculated as percentages of the control band intensity. Columns and bars represent the mean ± SEM of three or four separate experiments with four different culture preparations (n = 4). Statistical significance was determined by one-way ANOVA followed by Tukey’s tests: *p < 0.05 versus control