Fig. 5.

The Pac2 UbL domain interacts with the SCF complex. a ^GFPPac2 and ^GFPPac2ΔUbL were immunoprecipitated with anti-GFP from yeast extracts made from cells that expressed ^mycCdc53. The bead fractions were blotted with anti-myc antiserum to detect Cdc53 and with anti-GFP to detect immunoprecipitated ^GFPPac2. b ^GSTSkp1-Cdc53 heterodimers (SC) from Sf9 cells on GSH beads or GST peptides were incubated with yeast extracts from pac2Δ mutants expressing pGFP-PAC2 or pGFP-PAC2ΔUBL. Interaction was detected by Western blot with anti-GFP antibodies. c Half-life of Pac2 in wild-type cells and in cdc53 mutants measured at 37°C. Experimental details as in Fig. 4c