Fig. 2.
RBC bioactivities are independent of major RBC membrane proteins. PBLs (1.5 × 106) labeled with CFSE were stimulated with 5 μg/ml of PHA and cultured for 7 days either alone or in the presence of untreated RBC or RBC treated with PLC, anti-LFA-3/CD58 and neuraminidase. Afterward, cells were harvested and acquired in a FACSCalibur. The graph shows the percentage of proliferating PBLs (mean ± SEM, n = 7) as determined by CFSE fluorescence loss in the different conditions (**p < 0.01; *p < 0.05)