Fig. 5.

Inhibition of 3W mut-5LO activity by hyperforin. Human recombinant wt-5LO or mutated 3W mut-5LO (0.5 µg each) were expressed in E.coli, partially purified and pre-incubated in 1 ml PG buffer plus 1 mM EDTA with hyperforin, ZM230487, or BWA4C at the indicated concentrations or with vehicle (DMSO) at 4°C. ATP (1 mM) was added, and after 5–10 min, samples were prewarmed at 37°C for 30 s and 5-LO product formation was started by addition of 2 mM CaCl₂ and 20 µM AA. After another 10 min, 5-LO product formation was determined. Values are given as mean + SE, n = 3–4; **P < 0.01, *P < 0.05 versus WT-5LO at corresponding concentrations of inhibitors