Table 3.

Modulation of the transcript amounts of differentiation marker and PADI1–3 genes by NHK cell density

	NHK cell density
	Intermediate/low	High/low
KRT10	1.04 ± 0.16	8.90 ± 2.28**
FLG	1.02 ± 0.32	7.73 ± 2.57**
IVL	0.40 ± 0.32**	2.61 ± 2.72
LGALS 7	4.56 ± 2.17*	18.09 ± 12.49*
PADI1	0.69 ± 0.30*	3.49 ± 0.98**
PADI2	1.03 ± 0.34	0.72 ± 0.20*
PADI3	5.03 ± 1.62**	0.91 ± 0.19

Quantitative RT-PCR analysis of the steady-state levels of the indicated mRNAs was performed using NHKs harvested at low, intermediate and high cell density, and YWHAZ mRNA as the reference. The ratios are expressed as mean ± SEM for three independent experiments, each performed in duplicate plates. Abbreviations are as described in the legend of Table 1

*P < 0.05, **P < 0.001