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. 2024 May 23;15:4350. doi: 10.1038/s41467-024-48555-z

Fig. 1. Clade 2.3.4.4b H5 HA mRNA-LNP vaccine elicits long-lasting antibody responses in mice.

Fig. 1

Five mice were included per experimental group. Mice were vaccinated i.m. with 1 or 10 μg A/Astrakhan/3212/2020 HA mRNA-LNP (H5 mRNA-LNP) or 10 µg Ovalbumin mRNA-LNP (control mRNA-LNP). Serum samples were collected from mice at 28, 100, and 365 days after vaccination and serum IgG reactive to the A/Astrakhan/3212/2020 recombinant full-length HA protein (A) or ‘headless’ H5 stalk protein (B) were quantified by ELISA. C A/Astrakhan/3212/2020 neutralizing antibodies were also quantified by a 50% Foci reduction neutralization test (FRNT50); reciprocal dilutions of serum required to inhibit 50% virus infection are shown. All data are shown as geometric means ± 95% confidence intervals. Data were compared using two-way ANOVA with Tukey’s multiple comparisons test. Values were log-transformed before statistical analysis. Data are representative of 2 independent experiments. A all *P < 0.0001; (B) Day 365 control vs. 1 µg H5 mRNA-LNP *P = 0.0009, all others *P < 0.0001; (C) Day 28 1 µg H5 mRNA-LNP vs. 10 µg H5 mRNA-LNP *P = 0.0015, day 100 1 µg H5 mRNA-LNP vs. 10 µg H5 mRNA-LNP *P = 0.0002, day 365 1 µg H5 mRNA-LNP vs. 10 µg H5 mRNA-LNP *P = 0.0018, all other comparisons *P < 0.0001.Source data are provided as a Source Data file.