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. 2024 May 23;15:4350. doi: 10.1038/s41467-024-48555-z

Fig. 2. Clade 2.3.4.4b H5 HA mRNA-LNP vaccine elicits antibodies that bind and neutralize different clade 2.3.4.4b H5 viruses.

Fig. 2

Five mice were included per experimental group. Mice were vaccinated i.m. with 1 or 10 μg A/Astrakhan/3212/2020 HA mRNA-LNP (H5 mRNA-LNP) or 10 µg Ovalbumin mRNA-LNP (control). A, B Serum samples collected 28 days after vaccination were tested by ELISA to quantify IgG reactive to A/red fox/England/AVP-M1-21-01/2020 or A/pheasant/New York/22-009066-001/2022 recombinant HA proteins. C, D A/red fox/England/AVP-M1-21-01/2020 and A/pheasant/New York/22-009066-001/2022 neutralizing antibodies were quantified by 50% Foci reduction neutralization test (FRNT50) using serum samples collected 28 days after vaccination; reciprocal dilution of serum amounts required to inhibit 50% virus infection are shown. All data are shown as geometric means ± 95% confidence intervals. Data were compared using one-way ANOVA with Tukey’s post hoc test. Values were log-transformed before statistical analysis. Data are representative of 2 independent experiments. *P < 0.0001. Source data are provided as a Source Data file.