Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 23;15:4350. doi: 10.1038/s41467-024-48555-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Groups of five mice were vaccinated i.m. with 1 or 10 µg A/Astrakhan/3212/2020 HA mRNA-LNP (H5 mRNA-LNP) or 10 µg Ovalbumin mRNA-LNP (control). Serum samples were collected 28 days after vaccination and serum antibody binding to HA proteins from diverse of H5 viral strains was determined using a bead-based multiplex binding assay. We measured antibody binding to the HA of (A) A/Astrakhan/3212/2020, (B) A/red fox/England/AVP-M1-21-01/2020, (C) A/pheasant/NY/22-009066-001/2022, (D) A/Vietnam/1203/2004, (E) A/Hubei/1/2010, and (F) A/Indonesia/5/2005. Binding is reported as mean fluorescence intensity (MFI) values. All data are shown as geometric means ± 95% confidence intervals. Data were compared using two-way ANOVA with Tukey’s multiple comparisons test. Values were log-transformed before statistical analysis. Data are representative of 2 independent experiments. A 1 µg vs. 10 µg *P = 0.004; (B) 1 µg vs. 10 µg *P = 0.0065; (E) 1 µg vs. 10 µg *P = 0.0002; all other comparisons *P < 0.0001. Source data are provided as a Source Data file.