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. 2024 May 23;15:4350. doi: 10.1038/s41467-024-48555-z

Fig. 4. Clade 2.3.4.4b H5 HA mRNA-LNP vaccine elicits robust CD8+ T cell responses.

Fig. 4

Five mice per group were vaccinated i.m. with 1 μg A/Astrakhan/3212/2020 HA mRNA-LNP (H5 mRNA-LNP) or 1 μg Ovalbumin mRNA-LNP (control). Spleens were harvested at 10 days after vaccination and splenocytes were incubated with H5 HA overlapping peptide pools before completing intracellular cytokine staining and flow cytometric analysis of CD8 T cells. A Representative gating strategy for analysis of cytokine production by activated CD8+ T cells 10 days after vaccination. B Representative flow cytometry analysis of IFN-γ and TNF-α producing CD8+ T cells from mice vaccinated with control mRNA-LNP or H5 HA mRNA-LNP. C, D Number of IFN-γ producing CD8+ T cells and IFN-γ and TNF-α producing CD8+ cells were quantified. Data were compared using an unpaired two-tailed t test. Data are representative of 2 independent experiments. C *P = 0.0023; (D) *P = 0.0005. Source data are provided as a Source Data file.