Table 1.
Information on the antibodies that were used for T cell assays
| Antibody | Catalog # | Clone # | Manufacturer | Fluorophore | Dilution |
|---|---|---|---|---|---|
| IFNg | 11-7311-41 | XMG1.2 | Ebioscience | FITC | 1:400 |
| Foxp3 | 35-5773-82 | FJK-16s | Ebioscience | PECy5.5 | 1:200 |
| CXCR5-biotin | 13-7185-82 | SPRCL5 | Ebioscience | n/a | 1:50 |
| Streptavidin | 405237 | n/a | Biolegend | AF647 | 1:500 |
| CD90.2 | 140324 | 53-2.1 | Biolegend | AF700 | 1:400 |
| Ghostdye Red 780 | 13-0865-T100 | n/a | Tonbo | n/a | 1:500 |
| KLRG1 | 740279 | 2F1 | BD | BUV395 | 1:200 |
| CD4 | 612952 | GK1.5 | BD | BUV496 | 1:400 |
| CD8a | 752637 | 5H10-1 | BD | BUV563 | 1:400 |
| CD122 | 741537 | 5H4 | BD | BUV661 | 1:200 |
| CD69 | 612793 | H1.2F3 | BD | BUV737 | 1:200 |
| CD11a | 741919 | 2D7 | BD | BUV805 | 1:500 |
| TNFa | 506328 | MP6-XT22 | Biolegend | BV421 | 1:300 |
| PD-1 | 135220 | 29 F.1A12 | Biolegend | BV605 | 1:200 |
| CD19 | 115541 | 6D5 | Biolegend | BV650 | 1:400 |
| CD62L | 104445 | MEL-14 | Biolegend | BV711 | 1:400 |
| CD44 | 103059 | IM7 | Biolegend | BV785 | 1:400 |
| IL-4 | 504104 | 11B11 | Biolegend | PE | 1:200 |
| Bcl-6 | 562401 | K112-91 | BD | PE-CF594 | 1:50 |
| T-bet | 15-5825-82 | 4B10 | Ebioscience | PECy5 | 1:200 |
| CD107a | 121620 | 1D4B | Biolegend | PECy7 | 1:400 |