Table 2.
Operating conditions of GC–MS/MS.
| Chromatographic parameters | |
|---|---|
| Liner | Ultra Inert Liner of 78.5 mm, ID 4 mm, OD 6.47 mm, volume 900 μL and single taper (Agilent technologies Little Falls, DE, United States) |
| Column 1 and Column 2 | Ultra Inert 15 m x 0.25 mm, 0.25 μm film thickness (Agilent J&W. Scientific, Folsom, United States) |
| Carrier gas and flow mode | Helium carrier gas (99.999%); Flow in Colum 1: 1.0 mL/min Flow in Colum 2: 1.2 mL/min |
| Injection mode, temperature, and volume | Splitless mode, 280°C, 1 μL, hold time 3.0 min |
| Oven temperature | 60°C (held 1.0 min); 170°C (rate 40°C/min, held 3.75 min) and 310°C (rate 10°C/min) |
| Blackflush | 320°C (held 5.0 min) |
| Run time | 18 min + 5 min blackflush |
| Mass spectrometer parameters | |
|---|---|
| Detector | MS operating in electron ionization mode: 70 eV |
| Temperature of MS1 and MS2: 150°C | |
| Source temperature: 280°C | |
| Transfer line: 290°C | |
| Acquisition in Multiple Reaction Monitoring mode (MRM), | |
| Resolution MS1 = 1.5 | |
| Resolution MS2 = 1.5 | |