Table 6.
Validation parameters of BAP, BAA, BBF, and CHR, determined by means of GC–MS/MS analysis of chicken samples, extracted and cleaned-up with Protocol VII.
| PAH | t sb/b < 0.22a | Intercept | R2b | LODc | LOQd | SD ± RSD %e | Recovery % ± RSD %e | Uncertainty %f |
|---|---|---|---|---|---|---|---|---|
| BAP | 0.056 | 1.803 × 10−3 | 0.999 | 0.036 | 0.110 | 0.004 ± 5.56 | 73.08 ± 4.06 | 10.15 |
| BAA | 0.035 | 3.295 × 10−3 | 0.999 | 0.023 | 0.070 | 0.005 ± 5.43 | 94.25 ± 5.12 | 9.98 |
| BBF | 0.043 | 1.457 × 10−3 | 0.999 | 0.028 | 0.080 | 0.005 ± 7.29 | 76.07 ± 5.54 | 12.57 |
| CHY | 0.030 | 2.604 × 10−3 | 0.999 | 0.019 | 0.060 | 0.009 ± 7.91 | 110.70 ± 8.75 | 13.47 |
a
t-Student of the ratios sb/b, where sb is the standard deviation of the slope of MMC curve (b).
b
R2is the coefficients of determination of MMC curve.
c
Limit of detection (LOD) = 3.3sa/b where sa was the standard deviation of the intercept.
d
LOQ = 10sa/b, both calculated from MMC curve.
e
Mean values ± standard deviations (n = 6) of blank matrices fortified at 0.1 μg/kg for each analyte.
f
Mean values determined of blank matrices fortified at 0.1 μg/kg for each analyte (n = 6).