Table 1.
Antibodies used for flow cytometry and immunofluorescence.
| Antibody | Fluorophore | Manufacturer, product No., dilution | Usage |
|---|---|---|---|
| Rat anti-mouse CD16/CD32 | – | BD Biosciences, 553142 | Fc block |
| Aqua Live/Dead | AmCyan | Thermo Fisher Scientific, 65-0866-14 | All flow cytometry |
| Rat anti-mouse CD45 | BUV396 | BD Biosciences, 564279 | Flow cytometry analysis |
| Mouse anti-mouse CD64 | BV786 | BD Biosciences, 741024 | Flow cytometry analysis |
| Rat anti-mouse CD11b | APC-Cy7 | BD Biosciences, 557657 | Flow cytometry analysis |
| Rat anti-mouse B220 | PE-CF594 | BD Biosciences, 562313 | Flow cytometry analysis |
| Rat anti-mouse CD4 | PE-CF594 | BD Biosciences, 562314 | Flow cytometry analysis |
| Rat anti-mouse CD8 | PE-CF594 | BD Biosciences, 562315 | Flow cytometry analysis |
| Rat anti-mouse Ly6G | PE-CF594 | BD Biosciences, 562700 | Flow cytometry analysis |
| Mouse anti-mouse NK 1.1 | PE-CF594 | BD Biosciences, 562864 | Flow cytometry analysis |
| Rat anti-mouse Siglec-F | PE-CF594 | BD Biosciences, 562757 | Flow cytometry analysis |
| Mouse anti-mouse Cx3cr1 | BV650 | BioLegend, 149033 | Flow cytometry analysis |
| Mouse anti-mouse H2-Kb | Alexa Fluor 700 | Thermo Fisher Scientific, 56-5958-82 | Flow cytometry analysis |
| Mouse anti-mouse H2-Db | PE-Cy7 | Thermo Fisher Scientific, 25-5999-82 | Flow cytometry analysis |
| Rat anti-mouse MHC II | PE | Thermo Fisher Scientific, 12-5321-82 | Flow cytometry analysis |
| Hamster anti-mouse CD11c | BV421 | BD Biosciences, 562782 | Flow cytometry analysis |
| Rat anti-mouse CD19 | PE | BD Biosciences, 553786 | Flow cytometry compensation |
| Rat anti-mouse CD19 | Alexa Fluor 700 | BD Biosciences, 557958 | Flow cytometry compensation |
| Rat anti-mouse CD8 | – | Invitrogen, 14-0081-85, 1:250 | Primary (IF) |
| Rat anti-mouse LCMV | – | Bio Cell, BE0106, 1:1000 | Primary (IF) |
| Rabbit anti-mouse IBA-1 | – | WAKO, 019-19741, 1:500 | Primary (IF) |
| Goat anti-mouse CD31 | – | R&D Systems, AF3628, 1:250 | Primary (IF) |
| Donkey anti-rat | Alexa Fluor 555 | Thermo Fisher Scientific, A78945, 1:500 | Secondary (IF) |
| Donkey anti-rabbit | Alexa Fluor 647 | Thermo Fisher Scientific, A31573, 1:500 | Secondary (IF) |
| Donkey anti-goat | Alexa Fluor 488 | Thermo Fisher Scientific, A48259, 1:500 | Secondary (IF) |