Figure 2.

Concomitant increase in osmotic stress-induced PtdIns(4,5)P₂ and IP₃ levels. Sorbitol (at a final concentration of 1 m) was added to myo-[2-³H]inositol-labeled plants and an equivalent number of plants were withdrawn at 0, 15, and 30 min. A, The elution profile of gPI(4,5)P₂ (elution volume of 41–46 mL) is shown. B, The elution profile of inositol 1,4,5-trisphosphate (elution volume of 55–60 mL) is shown. For each HPLC run, the aqueous or organic sample each contained 1 × 10⁶ cpm.