Figure 5.

Calcium mobilization in root-tip cells. A, Root tips were loaded with Fluo-3 and were untreated (▴), treated with 0.25 m NaCl (▪), or treated with 0.25 m NaCl in the presence of the PLC inhibitor U-73122 (♦). NaCl was added at t = 0 and data for these experiments was collected simultaneously from plants laying adjacent to each other. For the untreated experiment (▴), 100 μL of buffer was added at t = 0. B, Mean and sd calculations of root tip cell fluorescence (n = 9 for each treatment). Roots were treated as described above, images collected, and relative fluorescence presented; control (black), salt stressed (light gray), and salt stressed plus U-73122 (white). Salt stressed indicates exposure to 0.25 m NaCl. C, Mean and sd calculations of fluorescence ratios using Fura-2-loaded 8-d-old root tips (n = 7 for each treatment). Root tips were untreated (black) or were treated with 0.25 m NaCl (light gray) or 10 μm 4-Bromo A-23187 (dark gray; as a positive control).