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. 2024 May 10;15:1395945. doi: 10.3389/fimmu.2024.1395945

Figure 3.

Figure 3

Donor APC and cDC depletion demonstrate diverse impacts on two major subsets of DCs, following murine skin and hind limb transplantation. Immune cells were isolated from blood and lymphoid tissues (spleen and lymph nodes) of the recipients (DBA/2 mice) of skin and hind limb grafts-derived from untreated (WT), APC depleted (CL; clodronate treated) and cDC depleted (zDC; DT treated zDC-DTR) donor mice at POD 6 and analyzed by flow cytometry. The bar graphs indicate frequencies of two major subsets of cDCs, cDC1 (CD11b-B220-CD11c+CD8+) (A–C) and cDC2 (CD11b+CD11c+MHCII+) (D–F) in the specified organs. The cDCs were gated on recipient-specific H2Kd+ CD45+ cells. The gating strategies are described in Supplementary Figure 3 . All values are given in % of the described population on the ordinate/”y-axis”. (n=8 skin Tx, n=5 hind limb Tx). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.