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. 2024 May 10;15:1395945. doi: 10.3389/fimmu.2024.1395945

Figure 4.

Figure 4

Effect of donor APC and cDC depletion on recipient T lymphocytes, following murine skin and hind limb transplantation. Immune cells were isolated from blood, lymphoid tissues (spleen and lymph nodes) and the allografts of the recipients (DBA/2 mice) of skin and hind limb grafts-derived from untreated (WT), APC depleted (CL; clodronate treated) and cDC depleted (zDC; DT treated zDC-DTR) donor mice at POD 6 and analyzed by flow cytometry. The bar graphs indicate frequencies of two major subsets of T lymphocytes, Th (CD3+CD4+) (A–C) and CTLs (CD3+CD8+) (D–F) and active Th cells (CD3+CD4+CD25+) (G–I) isolated from spleen, blood and lymph nodes of the recipients. (J, K) The bar graphs indicate frequencies of recipient CD8+ (J) and CD4+ (K) T cells infiltrated in the skin and hind limb grafts of the recipients at POD 6 in untreated (WT), APC depleted (CL) and cDC depleted (zDC) groups. The lymphocytes were gated on recipient-specific H2Kd+ CD45+ cells. The gating strategies are described in Supplementary Figure 2 . All values are given in % of the described population on the ordinate/”y-axis”. (n=8 skin Tx, n=5 hind limb Tx). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.