Table II.
C6 oxidation of BRs by AtBR6ox expressed in yeast
| Substrate | Product | Conversion Ratea
|
||
|---|---|---|---|---|
| First experiment | Second experiment | Vector | ||
| % | ||||
| [2H6]6-DeoxoCS | [2H6]CS | 35 | 42 | N.D.b |
| [2H6]6-DeoxoCS | [2H6]6-OHCS | 16 | 13 | N.D. |
| [2H6]6-DeoxoTY | [2H6]TY | 23 | – | N.D. |
| [2H6]6-Deoxo3DT | [2H6]3DT | 36 | 38 | N.D. |
| [2H6]6-DeoxoTE | [2H6]TE | 13 | 21 | N.D. |
| [2H6]6-DeoxoCT | [2H6]CT | N.D. | N.D. | N.D. |
| [2H6]CN | [2H6]6-OxoCN | N.D. | N.D. | N.D. |
a
The conversion rate was calculated roughly as a percentage of the amount of each metabolite versus the amount of substrate added to the culture.
b
N.D., Not detected.