REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
Antibodies | ||
Mouse monoclonal anti-Otoferlin antibody (HCS-1) |
DSHB |
Cat.#HCS-1; RRID:AB_10804296 |
Alexa fluor-674 goat anti-mouse antibody |
Thermo Fisher |
Cat.# A-21236; RRID:AB_2535805 |
Anti-Digoxigenin-AP, Fab Fragments |
Millipore Sigma |
Cat.# 11093274910; RRID:AB_2734716 |
Chemicals, peptides, and recombinant proteins | ||
(2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) (DASPEI) |
BIOTIUM |
70018 |
DAPI |
Thermo Fisher |
D1306 |
Neomycin sulphate (NEO) |
Millipore Sigma |
N0400000 |
Digoxygenin RNA labeling mix |
Millipore Sigma |
11277073901 |
Experimental models: Organisms/strains | ||
Zebrafish: Wildtype *AB |
ZIRC http://zebrafish.org |
ZFIN:ZDB-GENO-960809-7 |
Zebrafish: epcam jh79 |
ZFIN:ZDB-ALT220225-4 | |
Oligonucleotides | ||
epcam in situ hybridization probe template primers Forward:5’GGTTCCCAAGTGCTTCCTCA3’ Reverse:5’CCAAGCTTCTAATACGACTCACTATAGGGAGACAGCACAACTGCCCCAATTT3’ |
IDT |
ZFIN:ZDB-GENE-040426-2209 |
Software and algorithms | ||
FIJI |
https://imagej.nih.gov/ij/ |
|
PRISM |
GraphPad |
www.graphpad.co |