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. 2024 May 8;2024:10.17912/micropub.biology.001219. doi: 10.17912/micropub.biology.001219

REAGENT or RESOURCE

SOURCE

IDENTIFIER

Antibodies

Mouse monoclonal anti-Otoferlin antibody (HCS-1)

DSHB

Cat.#HCS-1; RRID:AB_10804296

Alexa fluor-674 goat anti-mouse antibody

Thermo Fisher

Cat.# A-21236; RRID:AB_2535805

Anti-Digoxigenin-AP, Fab Fragments

Millipore Sigma

Cat.# 11093274910; RRID:AB_2734716

Chemicals, peptides, and recombinant proteins

(2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) (DASPEI)

BIOTIUM

70018

DAPI

Thermo Fisher

D1306

Neomycin sulphate (NEO)

Millipore Sigma

N0400000

Digoxygenin RNA labeling mix

Millipore Sigma

11277073901

Experimental models: Organisms/strains

Zebrafish: Wildtype *AB

ZIRC http://zebrafish.org

ZFIN:ZDB-GENO-960809-7

Zebrafish: epcam jh79

(Edelman et al., 2021)

ZFIN:ZDB-ALT220225-4

Oligonucleotides

epcam in situ hybridization probe template primers

Forward:5’GGTTCCCAAGTGCTTCCTCA3’

Reverse:5’CCAAGCTTCTAATACGACTCACTATAGGGAGACAGCACAACTGCCCCAATTT3’

IDT

ZFIN:ZDB-GENE-040426-2209

Software and algorithms

FIJI

(Schindelin et al., 2012)

https://imagej.nih.gov/ij/

PRISM

GraphPad

www.graphpad.co