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. 2024 May 10;11:1393977. doi: 10.3389/fvets.2024.1393977

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Copyright © 2024 Felipo-Benavent, Valls, Monteiro, Jávega, García-Párraga, Rubio-Guerri, Martínez-Romero and O’Connor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative analysis of Annexin-V-FITC binding and FSC decrease in whole blood platelets after activating Bottlenose dolphin platelets in a whole blood sample with calcium ionophore A23187. Five regions (A–E) were defined over the Time axis in Annexin V-FITC/Time (A) and FSC/Time kinetic plots (B) and the mean intensity values obtained with the CytExpert software. The resulting values were represented in standard kinetic graphs displaying in arbitrary units (Y-axes) the mean fluorescence intensity (Annexin-V-FITC) (C) and the mean scatter intensity (Log FSC) variations along time (D).