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. 2001 Jun;126(2):849–860. doi: 10.1104/pp.126.2.849

Figure 5.

Figure 5

Induction of GSs and Trp/IGS pathway genes by MeJa. Three-week-old Col-0 wild-type as well as NahG, ein 2-1, and coi 1-1 plants were treated on leaves with 2 μL of MeJa (500 μm) or 0.1% (v/v) Tween 20 (control). A, Forty-eight h after treatment, leaves were harvested and the GSs IGS, 4-MIGS, and 4-MSBGS were quantified by HPLC. The data shown are the means ± se of four measurements. In each case the GS content was determined within five pooled plants. FW, Fresh weight of tissue. B, In experiments with Col-0 wild type, RNA gel blots were probed with ASA, TSA, TSB, CYP79B2, CYP79B3, and SGT at different time points after the addition of MeJa or 5 h after addition of 0.1% (v/v) Tween 20 as control (C). Membranes were reprobed with a ribosomal probe to ensure equal loading. C, RNA gel blots from NahG, ein2-1, and coi1-1 plants were probed with ASA, TSA, and CYP79B3 at different time points after the addition of MeJa or 5 h after mock treatment (C). Membranes were reprobed with a ribosomal probe to ensure equal loading.