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GS Induction by MeJa in Trp biosynthesis mutants. Three-week-old axenic trp2-8 and trp3-1 Trp mutant as well as Col-0 wild-type plants were treated on leaves with 2 μL MeJa (500 μm) or 0.1% (v/v) Tween 20 (control). Forty-eight h after treatment, leaves were harvested and the GSs IGS, 4-MIGS, and 4-MSBGS were quantified by HPLC. The data shown are the means ± se of three measurements. In each case the GS content was determined within five pooled plants.
