Table I.
Effect of GS-containing Arabidopsis extracts on the growth of E. carotovora subsp. carotovora SCC3193
| Extract | Myrosinase | IC50a | FLb |
|---|---|---|---|
| Col-0 | − | >60 | |
| Col-0 | + | 3.3 | (2.7–4.2) |
| ein2-1 | − | >60 | |
| ein2-1 | + | 1.6 | (0.8–3.5) |
| coi1-1 | − | >60 | |
| coi1-1 | + | 12.7 | (9.2–17.3) |
| Myrosinase | No inhibitory effect up to 10 units mL−1 | ||
Arabidopsis extracts from Col-0 wild type, as well as ein 2-1 and coi 1-1 mutant plants, were dried and resuspended in Luria-Bertani (LB) medium. During extraction, endogenous myrosinases were heat inactivated. Serial dilutions of the extracts were added to bacterial cultures suspended in 50 mm phosphate buffer (−) or in 50 mm phosphate buffer containing 1 unit mL−1 myrosinase (+).
a
The IC50 is the concentration, which resulted in 50% inhibition of bacterial growth after 15 h of incubation at 28°C. Values are in milligrams of extract (dry wt) per milliliter of LB medium.
b
FL, 95% Fiducial limits.