Table 2.
(A) Protective effect of apigenin on peroxynitrite-induced alterations in tryptophan residues of fibrinogen. (B) Protective effect of apigenin on peroxynitrite-induced alterations in tyrosine and tryptophan residues of fibrinogen.
| |||
|---|---|---|---|
| SN. | Comparative Assessment of Trp Residues | Trp-Fluorescence Alterations | % Protection by AP |
| 1. | Native Fibrinogen v/s ONOO−-Fibrinogen | 55.3% FI. decreased | - |
| 2. | AP50-ONOO−-Fibrinogen v/s Native Fibrinogen | 40.2% FI. decreased | 27.3 |
| 3. | AP50-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 25.2% FI. increased | - |
| 4. | AP75-ONOO−-Fibrinogen v/s Native Fibrinogen | 28.2% FI. decreased | 49.0 |
| 5. | AP75-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 37.7% FI. increased | - |
| 6. | AP100-ONOO−-Fibrinogen v/s Native Fibrinogen | 25.3% FI. decreased | 54.2 |
| 7. | AP100-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 44.0% FI. increased | - |
| |||
| SN. | Comparative Assessment of Tyr-Trp Residues | Tyr-Trp-Fluorescence Alterations | % Protection by AP |
| 1. | Native Fibrinogen v/s ONOO−-Fibrinogen | 51.6% FI. decreased | - |
| 2. | AP50-ONOO−-Fibrinogen v/s Native Fibrinogen | 24.8% FI. decreased | 50.9 |
| 3. | AP50-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 35.6% FI. increased | - |
| 4. | AP75-ONOO−-Fibrinogen v/s Native Fibrinogen | 22.4% FI. decreased | 56.6 |
| 5. | AP75-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 37.6% FI. increased | - |
| 6. | AP100-ONOO−-Fibrinogen v/s Native Fibrinogen | 9.8% FI. decreased | 81.0 |
| 7. | AP100-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 46.7% FI. increased | - |
Abbreviation: FI, fluorescence intensity; ONOO−, peroxynitrite; AP50, Apigenin at 50 µM; AP75, Apigenin at 75 µM; AP100, Apigenin at 100 µM; AP, Apigenin; Trp, tryptophan; Tyr, tyrosine.