Table 3.
Protective effect of apigenin on peroxynitrite-induced hydrophobic patches in fibrinogen.
| SN. | Comparative Assessment of Hydrophobicity | bis-ANS Binding Fluorescence | % Hydrophobic Protection by AP |
|---|---|---|---|
| 1. | Native Fibrinogen v/s ONOO−-Fibrinogen | 21.2% FI. increased | - |
| 2. | AP50-ONOO−-Fibrinogen v/s Native Fibrinogen | 13.0% FI. increased | 38.7 |
| 3. | AP50-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 9.3% FI. decreased | - |
| 4. | AP75-ONOO−-Fibrinogen v/s Native Fibrinogen | 8.8% FI. increased | 58.5 |
| 5. | AP75-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 13.6% FI. decreased | - |
| 6. | AP100-ONOO−-Fibrinogen v/s Native Fibrinogen | 8.2% FI. increased | 61.3 |
| 7. | AP100-ONOO−-Fibrinogen v/s ONOO−-Fibrinogen | 14.1% FI. decreased | - |
Abbreviation: bis-ANS, 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid; FI, fluorescence intensity; ONOO−, peroxynitrite; AP50, Apigenin at 50 µM; AP75, Apigenin at 75 µM; AP100, Apigenin at 100 µM; AP, apigenin.