Figure 3.

Complementation of the AS11 DGAT mutation with the WT cDNA leads to a restoration of WT seed oil composition and content. A, Transformation of Arabidopsis mutant line AS11 with single copies of the DGAT cDNA under the control of a napin promoter, leads to a restoration of the WT fatty acid composition in the seed oil of the transformant lines 3-3, 3-4, 9-1, 14-2, and 19-5. Fatty acid composition (% [w/w]) was determined on the seed oil extracted from Arabidopsis WT pSE and AS11 pSE (empty plasmid) control transformants, non-transformed (nt) WT and n-t AS11 controls, and T₃ seeds of napin:DGAT transgenic lines. B, Seed oil content of napin:DGAT T₃ transgenic AS11 mutant seed lines containing a single insertion of the DGAT cDNA. Oil content is expressed as percentage of seed dry weight for pSE in WT (empty plasmid) and n-t WT controls (stippled bars), for pSE in AS11 (empty plasmid) and n-t AS11 controls (black bars), and napin:DGAT AS11 transgenic lines 3-3, 3-4, 9-1,14-2, and 19-5 containing a single copy of the DGAT cDNA (gray bars). se bars are indicated (n = 3–5 replicate analyses performed on seed lots from each line with 100–200 seeds analyzed/replicate).