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Steroid-spiked curves in human urine compared with the respective curves in methanol measured using the GC-MS (A) and LC-HRMS (B) methods. F: cortisol, E: cortisone, 6βOHF: 6β-hydroxycortisol, 18OHF: 18-hydroxycortisol. Four replicates were obtained from distinct methanol samples and an additional four from separate serum sources.
