Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 4;13(5):320. doi: 10.3390/biology13050320

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

BACE1 functional activity towards human APP/Aβ is conserved in cnidarians but not in ctenophores or choanoflagellates. CHO 695 cells stably transfected with human APP were transiently transfected with the following cDNA expression constructs: GFP (negative control), Hydra BACE1, Mnemiopsis BACE 1/2, or Monosiga BACE 1/2. Homo BACE1 and Branchiostoma (Branch.) BACE1 constructs were used as positive controls. Conditioned media were harvested after 16 h, and the secretion of human Aβ was determined via ELISA. Data were normalized to total protein and analyzed with one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05; ** p < 0.01). Data are representative of three independent transfection rounds. Homo, Branchiostoma, and Hydra BACE1 all elevated Aβ secretion (A), while Mnemiopsis and Monosiga BACE 1/2 did not (B).

BACE1 functional activity towards human APP/Aβ is conserved in cnidarians but not in ctenophores or choanoflagellates. CHO 695 cells stably transfected with human APP were transiently transfected with the following cDNA expression constructs: GFP (negative control), Hydra BACE1, Mnemiopsis BACE 1/2, or Monosiga BACE 1/2. Homo BACE1 and Branchiostoma (Branch.) BACE1 constructs were used as positive controls. Conditioned media were harvested after 16 h, and the secretion of human Aβ was determined via ELISA. Data were normalized to total protein and analyzed with one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05; ** p < 0.01). Data are representative of three independent transfection rounds. Homo, Branchiostoma, and Hydra BACE1 all elevated Aβ secretion (A), while Mnemiopsis and Monosiga BACE 1/2 did not (B).