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The principles of the APPROACH strategy for exosomal biomarker detection. (A) The workflow of the APPROACH strategy. (B) Schematic details of the PLA, RCA, and TR−FRET steps. (C) The absorption spectra of Tb (black) and Cy5 (red), and the emission spectra of Tb (green) and Cy5 (orange). (D) The chemical structures of CoraFluor−like−NHS (Tb).
