Table 2.
Biosynthesis of PHA copolymers from CO2 by recombinant R. eutropha 1F2 harboring phaCAc_NSDG.
| Entry | Genome | Plasmid-Based Expression | Dry Cell wt. (g/L) |
PHA Content (wt.%) |
PHA (g/L) |
PHA Composition (mol%) a |
Mw (×105) b |
PDI b | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3HB | 3HV | 3H4MV | 3H2MP | ||||||||
| 7 | - | bktB, kivd, padA | 0.72 | 28.4 | 0.20 | 92.7 | 6.4 | 0.9 | 0 | 1.88 | 2.04 |
| 8 | ΔhbdH | - | 1.20 | 49.7 | 0.60 | 99.3 | 0 | 0.7 | trace | 3.56 | 2.24 |
| 9 | ΔhbdH | bktB | 1.14 | 49.0 | 0.55 | 97.4 | 0 | 1.2 | 1.4 | 0.59 | 1.64 |
The cells were cultivated in a 250 mL jar fermenter with 100 mL MS medium supplying a low-hydrogen-content gas mixture (3.8% H2, 7.3% O2, 13.0% CO2, and 75.9% N2) at 5 mL/min of gas flow rate, at 30 °C for 162 h [27]. a Determined by GC analysis. b Determined by GPC analysis. PDI, polydispersity index.