Figure 6.

Callose staining and immunogold labeling of spores using aniline blue and anti-(1-3)-β-glucan. A and B, Callose staining at different developmental stages in wild type (A) and gem1 (B), early bicellular (left), tricellular (middle), and mature pollen (right) stages. C and D, gem1 mature pollen grain showing exine splitting (arrows in C) and callose accumulation at the exposed intine region (arrows in D). E through G, Immunogold localization of (1-3)-β-glucan in wild type and gem1 at the late bicellular stage. E, Wild-type spore with no significant labeling in the intine wall. F, gem1 spore showing that the epitopes are mainly located at ectopic internal and intine walls. G, Epitopes are exclusively present in the wall region flanked by two junctions between intine and internal wall (indicated by arrows). L, Lipid body; V, vacuole. All scale bars = 0.5 μm.