Table 5.
Summary of stability assessment of Trp metabolite derivatives.
| Autosampler (n = 5) | Freeze–Thaw (n = 5) | |||||||
|---|---|---|---|---|---|---|---|---|
| Analyte | QC Level | Nominal c [ng/mL] | Found [ng/mL] | Accuracy [%] | RSD [%] | Found [ng/mL] | Accuracy [%] | RSD [%] |
| PA | LLOQ | 1 | 1.00 | 100.2 | 8.6 | 1.04 | 101.1 | 4.9 |
| Low | 2 | 2.05 | 102.4 | 9.8 | 2.17 | 108.6 | 1.0 | |
| Medium | 40 | 39.45 | 98.6 | 4.4 | 38.91 | 97.3 | 6.5 | |
| High | 80 | 79.07 | 98.8 | 0.6 | 79.55 | 99.4 | 1.4 | |
| NA | LLOQ | 5 | 5.11 | 102.3 | 10.2 | 5.19 | 103.9 | 14.3 |
| Low | 10 | 10.20 | 102.0 | 4.0 | 10.00 | 100.0 | 8.1 | |
| Medium | 200 | 207.19 | 103.6 | 3.8 | 188.04 | 94.0 | 8.1 | |
| High | 400 | 408.91 | 102.2 | 3.5 | 390.74 | 97.7 | 5.7 | |
| AA | LLOQ | 1 | 1.02 | 101.6 | 3.5 | 0.97 | 97.2 | 14.3 |
| Low | 2 | 2.03 | 101.2 | 3.3 | 1.92 | 95.9 | 8.6 | |
| Medium | 40 | 40.74 | 101.8 | 1.6 | 41.51 | 103.8 | 4.2 | |
| High | 80 | 80.62 | 100.8 | 3.3 | 85.85 | 107.3 | 7.5 | |
| 3-OH AA | LLOQ | 1 | 1.01 | 100.5 | 14.4 | 0.91 | 90.8 | 6.5 |
| Low | 2 | 1.99 | 99.4 | 6.8 | 2.05 | 102.3 | 9.7 | |
| Medium | 40 | 39.40 | 98.5 | 2.0 | 40.54 | 101.3 | 3.5 | |
| High | 80 | 80.58 | 100.7 | 2.8 | 81.43 | 101.8 | 4.0 | |
| KA | LLOQ | 1 | 1.01 | 101.2 | 7.3 | 0.95 | 95.2 | 9.8 |
| Low | 2 | 1.99 | 99.3 | 8.0 | 2.10 | 105.1 | 10.4 | |
| Medium | 40 | 42.21 | 105.5 | 1.5 | 41.70 | 104.2 | 7.8 | |
| High | 80 | 81.58 | 102.0 | 1.8 | 84.70 | 105.9 | 7.8 | |
| XA | LLOQ | 1 | 0.99 | 99.0 | 7.7 | 1.014 | 101.4 | 12.4 |
| Low | 2 | 2.033 | 101.6 | 8.1 | 1.897 | 94.9 | 6.3 | |
| Medium | 40 | 39.44 | 98.6 | 4.6 | 38.24 | 95.6 | 5.5 | |
| High | 80 | 78.24 | 97.8 | 2.4 | 77.71 | 97.1 | 2.5 | |
| QA | LLOQ | 10 | 10.17 | 101.7 | 5.9 | 10.16 | 101.6 | 9.4 |
| Low | 20 | 19.93 | 99.7 | 10.6 | 21.15 | 105.8 | 4.7 | |
| Medium | 400 | 405.13 | 101.3 | 2.4 | 395.47 | 98.9 | 3.8 | |
| High | 800 | 797.64 | 99.7 | 8.8 | 817.14 | 102.1 | 8.9 | |
| Trp | LLOQ | 500 | 506.88 | 101.4 | 7.5 | 492.62 | 98.5 | 14.2 |
| Low | 1000 | 1013.99 | 101.4 | 4.7 | 955.40 | 95.5 | 8.3 | |
| Medium | 20,000 | 20,438.98 | 102.2 | 2.5 | 19,603.80 | 98.0 | 7.4 | |
| High | 40,000 | 39,922.96 | 99.8 | 2.5 | 40,087.04 | 100.2 | 4.9 | |
| 3-OH KYN | LLOQ | 25 | 24.42 | 97.4 | 6.6 | 27.58 | 110.3 | 12.3 |
| Low | 50 | 50.70 | 101.4 | 3.4 | 53.02 | 106.0 | 6.0 | |
| Medium | 1000 | 1039.83 | 104.0 | 4.3 | 1033.51 | 103.4 | 3.7 | |
| High | 2000 | 2138.14 | 106.9 | 45.7 | 2239.98 | 112.0 | 5.5 | |
| I3LA | LLOQ | 10 | 10.38 | 103.7 | 7.0 | 9.57 | 95.7 | 12.0 |
| Low | 20 | 20.27 | 101.4 | 11.6 | 20.66 | 103.3 | 6.7 | |
| Medium | 400 | 405.01 | 101.3 | 1.3 | 429.28 | 107.3 | 4.6 | |
| High | 800 | 785.55 | 98.2 | 4.2 | 815.93 | 102.0 | 4.8 | |
| 5-OH IAA | LLOQ | 5 | 4.95 | 99.0 | 6.3 | 4.47 | 94.8 | 6.3 |
| Low | 10 | 9.02 | 90.4 | 6.6 | 10.98 | 109.8 | 11.8 | |
| Medium | 200 | 188.79 | 94.4 | 5.8 | 180.13 | 90.1 | 5.2 | |
| High | 400 | 388.75 | 97.2 | 14.0 | 373.21 | 93.3 | 8.9 | |