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Seahorse XF24 analyzer OCR measurements protocol
| Steps | Measurement loops |
|---|---|
| Sensor cartridge calibration | – |
| Equilibration | – |
| Baseline measurement | 2–3 cycles: Mix 3 min, Wait 2 min, Measure 3 min |
| Inject port A—oligomycin | – |
| Measure | 2–3 cycles: Mix 3 min, Wait 2 min, Measure 3 min |
| Inject port B—FCCP | – |
| Measure | 2–3 cycles: Mix 3 min, Wait 2 min, Measure 3 min |
| Inject port C—rotenone and antimycin A | – |
| Measure | 2–3 cycles: Mix 3 min, Wait 2 min, Measure 3 min |
