Table 6.

Physiochemical properties of the designed multi-epitope vaccine construct.

Physiochemical Features	Evaluation
Amino acid residue	367
Molecular weight	38.84 kDa
Theoretical PI	9.34
Total number of negatively charged residue (Asp + Glu)	28
Total number of positively charged residues (Arg + Lys)	44
Formula	C1737H2748N472O509S13
Extinction coefficients	97,000 M−1 Cm−1
Estimated half-life	30 h (mammalian reticulocytes, in vitro) >20 h (Yeast, in vitro) >10 h (Escherichiaa coli, in vivo)
Instability index	32.68 (stable)
Aliphatic index	75.59
Gravy	−0.377