FIG. 3.

Immunoblot assay of M. catarrhalis strain 87P15B1. All lanes contain purified outer membrane of M. catarrhalis strain 87P15B1. Lanes were probed with aliquots of homologous postclearance serum sample 87E16 as follows: a, unadsorbed; b, adsorbed with M. catarrhalis strain 87P15B1; c, adsorbed with E. coli; d, serum fraction eluted from the surface of M. catarrhalis strain 87P15B1; e, serum fraction eluted from E. coli. Antibodies were detected with peroxidase-conjugated anti-human IgG. The positions of molecular mass markers (in kilodaltons) are shown on the right. The arrows indicate bands to which antibodies were adsorbed (lane b) and/or eluted (lane d).