FIG. 5.

Immunoblot assay with postexacerbation serum sample 80E22 eluted from the surface of the homologous strain 80P20B1. Lanes contain purified outer membrane preparations of strains a) 80P20B1, b) O35E, c) UspA1 mutant, d) UspA2 mutant, e) UspA1 UspA2 double mutant, f) CopB mutant, g) TbpB mutant, h) Hag mutant, and i) UspA1 UspA2 Hag triple mutant. The positions of molecular mass markers (in kilodaltons) are shown on the left. The positions of UspA2, UspA1, and TbpB are indicated by arrows on the right.