FIG. 3.

Gene expression analysis of HUVEC after contact with PRBC. (A) RNA was isolated from RBC or PRBC-treated HUVEC and used for hybridization as described in Materials and Methods. The corresponding FDR value was 0.19417. Hierarchical clustering of genes identified by SAM as being statistically significantly differently expressed was performed using the Tiger software (version 2.2) with the average linkage clustering method (distance metric, Euclidean). (B) Results of gene expression profiling were validated at the protein level. Cell culture supernatants from HUVEC treated as described in the text were assayed for CCL20, CCL2, CXCL8, and pro-MMP-1 by specific ELISA. (C) Cell surface expression levels of CD44 and CD36 on HUVEC were determined by immunofluorescent staining and flow cytometric analysis.