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Mortality curves for rat pups infected with the wild-type strain NEM316 (○) and the SrtA− mutant NEM2135 (♦). Groups of 20 neonatal Sprague-Dawley rat pups (48 h old) were inoculated i.p. with 5 × 106 bacteria, and mortality was observed over a 7-day period. The difference in virulence of the two strains was considered not quite significant, with a two-tailed P value of 0.0849 by the Mann-Whitney test, as calculated with GraphPad Instat (version 3.0).
