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CpG ODN treatment induces IFN-γ production. C57BL/6 mice were injected i.p. with 50 μg of CpG ODN. (A) IFN-γ-secreting peritoneal cells were quantified on day 3 by enzyme-linked immunospot assay. (B) Serum IFN-γ levels were determined on day 0 and day 3 by enzyme-linked immunosorbent assay. Data represent the means ± SD of values for five to seven independently studied mice in three independent experiments. ***, P < 0.001 (two-tailed Student's t test).
