FIG. 4.

IFN-γ-dependent NO production following CpG treatment. C57BL/6 and IFN-γ KO mice were treated with 50 μg of CpG ODN. Peritoneal cells were isolated 3 days later and stimulated with HKL. (A) Effect of cytokine-specific neutralizing Abs on NO production. (B) Comparison of cells from WT, IFN-γ KO, and NOS2 KO mice. Data represent the means ± SD of values for six to nine independently studied mice in more than two independent experiments. ***, P < 0.001 versus values for all other groups (Student's t test). TNF-α, tumor necrosis factor alpha; IL-12, interleukin-12; IL-18, interleukin-18.