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. 2024 May 16;14(5):591. doi: 10.3390/biom14050591

Figure 2.

Figure 2

Analysis of TSG101 and LAMP1 protein expression in HEK293T cells post-transfection. (A,B) Western blot analysis showing the expression levels of TSG101 protein in HEK293T cells transfected with expression constructs for p53, ARP, or CDP, normalized to β-actin (n = 3 per group; * p < 0.05, ** p < 0.01). (C,D) Western blot analysis showing the expression levels of TSG101 protein in HEK293T cells transfected with expression constructs for p53, ARP, or CDP, normalized to GAPDH (n = 3 per group; *** p < 0.001). Original images can be found in Supplementary Materials. (E) Immunofluorescence staining illustrating the expression and localization level of the late endosomal marker LAMP1 (Red), with cell nuclei stained using DAPI (Blue). Compared to the p53 group, cells transfected with the expression constructs for ARP or CDP showed enhanced LAMP1 fluorescence signals. Scale bar = 20 µm.