FIG. 2.

Western immunoblot analysis of RvCFP and column chromatography protein pools using healthy tuberculin skin test-positive tuberculosis patient contact sera (A) and moderately advanced pulmonary tuberculosis patient sera (B). Polypeptides of each chromatography pool were separated by 16% SDS-PAGE, transferred on to nitrocellulose membrane (47), and probed with pooled sera of either moderately advanced active pulmonary TB patients (n = 10) or healthy Mantoux-positive tuberculosis contacts (n = 10). A representative blot is shown. UB, unbound.