TABLE 1.

Partial characterization of individual purified polypeptides (bands) eluted from different column chromatography pools

Protein pool and eluted protein no.	Apparent mol mass (kDa)a	Ab reactedb	Degree of reactivityc	Protein identified
Pool one
1	22.5	K8483	++
2	21	K8483	+++++	CFP-21
3	20	K8483	++
Pool two
4	3.5	Nil	−
5	4	Nil	−
6	4.5	Nil	−
7	5	Nil	−
8	6	Nil	−
9	8	Nil	−
10	10	IT-3	++
11	12	IT-3	++
12	14.5	α-MPT 53	+++++	15-kDa
13	16	Nil	−
14	16.5	Nil	−
15	18	Nil	−
16	19	IT-12	+++++
17	21	Nil	−
18	23.5	Nil	−
19	25	Nil	−
20	26	Nil	−
21	28	Nil	−
22	35	Nil	−
23	37	Nil	−
Pool three
24	18.5	IT-10/IT-12	+++
25	19	Nil	−
26	20	Nil	−
27	20.5	IT-10	++++	20.5-kDa
28	24	L24B4	+++++	MPT64
29	28	Nil	−
30	29	Nil	−
31	29.5	IT-49	+++++	Ag85B?
32	30.5	IT-49	+++++	Ag85A?
33	31	Nil	−
34	34	Nil	−
Pool four
35	4	PV-2	++
36	4.5	PV-2	+++++
37	5.5	PV-2 &	++	HYB 76-8
38	6	HYB 76-8	+++++	ESAT-6
39	7	HYB 76-8	++
40	8	Nil	−
41	9	K8493	++
42	9.5	K8493	+++
43	10	K8493	+++++	CFP-10
44	11	IT-3	++
45	12.5	IT-3	++
46	13.5	Nil	−
47	15	α-MPT-53	++
48	15.5	Nil	−
49	16	Nil	−
50	16.5	Nil	−
51	17	Nil	−
52	17.5	Nil	−
53	18	Nil	−
54	18.5	Nil	−
55	19.5	Nil	−
56	20	Nil	−
57	23	CS-28	+++++
58	25	Nil	−
59	26	IT-52	++++	MPT-51
60	27	Nil	−
61	28.5	Nil	−
62	33	Nil	−
63	36	Nil	−
Pool five
64	5	Nil	−
65	5.5	Nil	−
66	6	Nil	−
67	7	Nil	−
68	8	IT-3	++
69	9	IT-3	+++
70	10	IT-3	+++++	HSP-10
71	11	IT-3	+++
72	12	IT-3	+++
73	13	α-MPT-53	+
74	13.5	α-MPT-53	+
75	14	α-MPT-53	++
76	14.5	α-MPT-53	++
77	16	Nil	−
78	17	Nil	−
79	18	Nil	−
80	19	IT-69	++++
81	20	Nil	−
82	21	Nil	−
83	21.5	Nil	−
84	22	Nil	−
85	22.5	Nil	−
86	23	Nil	−
87	23.5	Nil	−
88	25	Nil	−
89	26.5	Nil	−
90	27	Nil	−
91	28	Nil	−
92	29	Nil	−
93	30	Nil	−
94	32	IT-44	+++++
95	34	IT-44	+++
96	35	IT-44	++
97	36	Nil	−
98	38	Nil	−
99	39	Nil	−
100	42	Nil	−
101	43	Nil	−
Pool eightd
102	16	IT-20	+++++	α-crystallin
103	26	mc-9246	++++	28-kDa
104	33	IT-59	++++	33-kDa

^aMolecular mass was determined by SDS-PAGE analysis.

^bAb, antibody.

^cReactivity (OD₄₉₂) for MAb/PAb. −, OD of <0.10 (nil); +, OD of 0.10 to 0.20; ++, OD of 0.20 to 0.30; +++, OD of 0.30 to 0.40; ++++, OD of 0.40 to 0.50; +++++, OD of >0.50.

^dThe low-molecular-mass polypeptides resolved in pools six, seven, and eight were apparently identical.