Figure 5. Astrocytes dynamically respond to sub-chronic stress, knockdown of aquaporin-4 leads to astrocyte dystrophy in the prefrontal cortex.

A) Representative images of vascular tomato lectin (magenta) and immunohistology for astrocyte GFAP (cyan) and AQP4 (yellow) in the PFC (20×, scale bar = 100 μm). B) Left: Representative images of immunohistology for ALDH1L1 in the PFC (20×, scale bar = 100 μm). Right: Number of ALDH1L1+ astrocytes in the PFC per mm². C) Area of GFAP+ material in the PFC. D) Area of GFAP+ material associated with blood vessels in the PFC. E) Total area of AQP4+ material in the PFC. F) Area of astrocyte AQP4+ material relative to vessel area. G) Area of tomato lectin+ vessels in the PFC. ^# p<0.05 main effect (no specific contrasts detected). * p<0.05 planned comparison indicated (Sidak’s test).