TABLE 2.
Evaluation of LCRS components as immunogenic and protective proteinsa
| Protein | Present in microarray | Immunogenic
|
Protective efficacy in bubonic/pneumonic model (reference[s]) | |
|---|---|---|---|---|
| Previous study | Present study | |||
| LcrV | Yes | Yes | Yes | Bubonic and pneumonic—protective (22, 35) |
| YopD | Yes | Yes | Yes | Bubonic—partially protective (2) |
| YscF | Yes | Yes | Yes | Bubonic—not protective (17) |
| VirG | Yes | Yes | Yes | Bubonic—not protective (17) |
| YopN | Yes | Yes | No | Bubonic—not protective (2, 21) |
| YscO | Yes | Yes | No | Bubonic—not protective (17) |
| YscP | Yes | Yes | No | Bubonic—not protective (17) |
| TyeA | Yes | Yes | No | Bubonic—not protective (17) |
| YopH | No | Yes | ND | Bubonic—not protective (2) |
| YopE | No | Yes | ND | Bubonic—not protective (2, 21) |
| YopK | No | Yes | ND | Bubonic—not protective (2, 21) |
| YopM | No | Yes | ND | Bubonic—not protective (2, 21) |
| YpkA | No | Yes | ND | Bubonic—delayed time to death (2) |
| YscJ | No | Yes | ND | Bubonic—not protective (17) |
| YscB | Yes | ND | Yes | ND |
| YscE | Yes | ND | Yes | ND |
| YscL | Yes | ND | Yes | ND |
| LcrG | Yes | ND | Yes | ND |
| SycE | Yes | ND | Yes | ND |
a
ND, not determined. A total of 10 Yop proteins were actually subjected to cloning and expression. The genes yopB and yopM could not be successfully amplified with Y. pestis 82009 DNA as a template. YopE, YopK, YopJ, and YopH were successfully expressed, but unfortunately, after being purified through 96-well format, they could not pass the quality control process. Therefore, only YopD, YopN, and YopR were included in the final version of the protein microarrays.