TABLE 1.

B. anthracis RA3 (pXO1⁺ pXO2⁺)-secreted proteins identified by MALDI-MS

Spot no.a	Protein name	Accession no.b	Signal peptidec	pId		Masse		Gene locationf
				Exptl	Theoretical	Exptl	Theoretical
Proteins identified directly by MS in RA3
1	EA1	NP_654830	+	5.79	5.70	102.6	91.4	Chr
2	EA1	NP_654830	+	5.70	5.70	101.8	91.4	Chr
3	Sap	NP_654829	+	6.42	6.89	83.4	86.7	Chr
4	Oligopeptide ABC transporter	NP_843667	+	6.39	6.56	58.5	61.8	Chr
5	Oligopeptide ABC transporter41	NP_843667	+	6.25	6.34	57.6	61.7	Chr
6	Oligopeptide ABC transporter	NP_843667	+	6.39	6.56	57.6	61.8	Chr
7	Protective antigen precursor A41	AAA22637	+	5.44	5.88	54.0	85.9	pXO1
8	Protective antigen precursor A	AAA22637	+	5.56	5.88	53.8	85.9	pXO1
9	Protective antigen precursor Ag	AAA22637	+	5.83	5.88	53.7	85.9	pXO1
10	Enolase	NP_653583	−	6.90	4.66	49.9	46.6	Chr
11	Enolase	NP_653583	−	4.67	4.66	50.4	46.6	Chr
12	Enolase41	NP_653583	−	4.79	4.66	50.4	46.6	Chr
13	S-layer homology domain	NP_657198	+	5.29	5.84	42.0	40.6	Chr
14	S-layer homology domain	NP_657198	+	5.40	5.84	41.9	40.6	Chr
15	Edema factor (EF)41g	AAA22374	+	5.36	6.95	36.6	92.5	pXO1
16	Edema factor (EF)gh	CAC93925	+	5.23	6.95	34.8	92.5	pXO1
17	Triosephosphate isomerase	NP_653585	−	5.15	5.00	28.4	26.7	Chr
18	Lethal factor (LF)41gh	AAA22569	+	4.75	5.62	28.9	93.9	pXO1
19	Zinc uptake system protein, ZnuAg	NP_052826	+	5.75	5.69	27.4	27.9	pXO1
20	Hypothetical proteinh	NP_653819	+	4.75	5.06	27.7	23.2	Chr
21	Zinc uptake system protein, ZnuAg	NP_052826	+	5.63	5.69	27.5	27.9	pXO1
22	Putative nucleoside diphosphate kinase	NP_655416	−	5.46	5.24	16.5	16.7	Chr
Proteins identified by positional overlapi
23	Sap	NP_654829	+	6.10	6.89	100.1	86.7	Chr
36	Sap	NP_654829	+	6.24	6.89	95.8	86.7	Chr
24	Sap	NP_654829	+	6.43	6.89	94.9	86.7	Chr
39	Phosphoglycerate mutase	NP_834804	−	4.90	4.86	64.1	56.9	Chr
40	Phosphoglycerate mutase	NP_981532	−	4.85	4.84	62.4	53.4	Chr
41	EA1	NP_654830	+	5.90	5.70	58.4	91.4	Chr
25	MerA mercuric reductase	CAA70224	−	5.66	5.30	54.1	58.8	Chr
42	Isocitrate lyase	NP_830914	−	6.02	5.27	52.0	47.1	Chr
11	Enolase	NP_653583	−	4.67	4.66	50.8	46.6	Chr
44	Enolase	NP_653583	−	4.72	4.66	50.3	46.6	Chr
26	Phosphoglycerol transferase	NP_834895	−	5.25	5.37	49.3	44.9	Chr
27	Cell surface protein A	NP_658575	+	5.19	5.56	48.6	99.9	Chr
28	Alanine dehydrogenase	NP_658654	−	5.48	5.22	46.6	40.3	Chr
29	Glyceraldehyde 3-phosphate dehydrogenase	NP_834805	−	5.70	5.04	44.0	35.5	Chr
45	Fructose-1,6-bisphosphatase	NP_847733	−	5.85	5.53	41.8	34.2	Chr
31	Malate dehydrogenase	NP_847040	−	5.38	5.12	42.2	33.6	Chr
32	Cysteine Synthase	NP_842636	−	5.64	5.37	41.1	33.0	Chr
47	EA1	NP_654830	+	6.06	5.7	35.5	91.4	Chr
34	Triosephosphate isomerase	NP_653585	−	5.07	5.13	29.3	26.7	Chr
49	Alkyl hydroperoxide reductase	NP_654276	−	4.73	4.81	24.2	20.9	Chr

^aSpot numbers refer to Fig. 1.

^bAccession numbers available through National Center for Biotechnology Information.

^cPresence of signal peptide predicted by SignalP 3.0 (+, yes; −, no).

^dIsoelectric point. Exptl, experimental.

^eMolecular Mass (kDa). Exptl, experimental.

^fChr, chromosome.

^gUnique to RA3 compared to RA3:00.

^hUnique to RA3 compared to RA3R.

ⁱOf proteins identified from RA3R and RA3:00 by MS.