TABLE 2.

B. anthracis RA3R (pXO1⁺ pXO2⁻)-secreted proteins identified by MALDI-MS

Spot no.a	Protein name	Accession no.b	Signal peptidec	pId		Masse		Gene location
				Exptl	Theoretical	Exptl	Theoretical
Proteins identified directly by MS in RA3R
1	EA1	NP_654830	+	5.79	5.70	102.6	91.4	Chrf
2	EA1	NP_654830	+	5.70	5.70	101.8	91.4	Chr
23	Sap	NP_654829	+	6.08	6.89	95.1	86.7	Chr
24	Sap	NP_654829	+	6.43	6.89	96.0	86.7	Chr
25	MerA mercuric reductase	CAA70224	−	5.64	5.30	52.9	58.8	Chr
26	Phosphoglycerol transferase	NP_834895	−	5.28	5.37	47.8	44.9	Chr
27	Cell surface protein A	NP_658575	+	5.19	5.56	44.5	99.9	Chr
28	Alanine dehydrogenase	NP_658654	−	5.47	5.40	44.6	40.2	Chr
29	Glyceraldehyde 3-phosphate dehydrogenase	NP_834805	−	5.71	5.04	44.0	35.5	Chr
13	S-layer homology domain	NP_657198	+	5.28	5.84	40.3	40.6	Chr
30	S-layer homology domain	NP_657198	+	5.28	5.84	39.7	40.6	Chr
31	Malate dehydrogenase	NP_847040	−	5.33	5.12	40.4	33.6	Chr
32	Cysteine Synthase	NP_842636	−	5.62	5.37	39.3	33.0	Chr
33	Putative lipoprotein, VanW-related	NP_845858	+	6.66	6.85	31.2	32.6	Chr
34	Triosephosphate isomerase	NP_653585	−	5.07	5.13	29.4	26.7	Chr
35	S-layer protein A	NP_652888	+	6.12	9.16	25.9	76.3	pXO1
Proteins identified by positional overlapg
36	Sap	NP_654829	+	6.26	6.89	87.3	86.7	Chr
24	Sap	NP_654829	+	6.45	6.89	92.2	86.7	Chr
3	Sap	NP_654829	+	6.40	6.89	76.1	86.7	Chr
39	Phosphoglycerate mutase	NP_834804	−	5.00	4.86	64.7	56.9	Chr
40	Phosphoglycerate mutase	NP_981532	−	4.89	4.84	62.4	53.4	Chr
4	Oligopeptide ABC transporter	NP_843667	+	6.22	6.56	58.5	61.8	Chr
6	Oligopeptide ABC transporter	NP_843667	+	6.30	6.56	56.8	61.8	Chr
5	Oligopeptide ABC transporter	NP_843667	+	6.40	6.34	56.9	61.7	Chr
41	EA1	NP_654830	+	5.94	5.70	57.1	91.4	Chr
7	Protective Antigen Precursor A	AAA22637	+	5.41	5.88	53.4	85.9	pXO1
8	Protective Antigen Precursor A	AAA22637	+	5.52	5.88	53.4	85.9	pXO1
9	Protective Antigen Precursor A	AAA22637	+	5.86	5.88	51.2	85.9	pXO1
42	Isocitrate Lyase	NP_830914	−	6.05	5.27	50.9	47.1	Chr
12	Enolase	NP_653583	−	4.87	4.66	48.6	46.6	Chr
11	Enolase	NP_653583	−	4.71	4.66	48.4	46.6	Chr
44	Enolase	NP_653583	−	4.78	4.66	48.1	46.6	Chr
10	Enolase	NP_653583	−	6.84	4.66	47.4	46.6	Chr
14	S-layer homology domain	NP_657198	+	5.41	5.84	38.8	40.6	Chr
45	Fructose-1,6-bisphosphatase	NP_847733	−	5.88	5.53	38.4	34.2	Chr
31	Malate dehydrogenase	NP_658620	+	5.34	5.12	39.5	33.6	Chr
17	Triosephosphate isomerase	NP_653585	−	5.12	5.00	27.6	26.7	Chr
22	Putative nucleoside diphosphate kinase	NP_655416	−	5.49	5.24	15.7	16.7	Chr

^aSpot numbers refer to Fig. 2A.

^bAccession numbers available through National Center for Biotechnology Information.

^cPresence of signal peptide predicted by SignalP 3.0 (+, yes; −, no).

^dIsoelectric point. Exptl, experimental.

^eMolecular mass (kDa). Exptl, experimental.

^fChr, chromosome.

^gOf proteins identified from RA3 and RA3:00 by MS.