TABLE 3.

B. anthracis RA3:00 (pXO1⁻ pXO2⁻)-secreted proteins identified by MALDI-MS

Spot no.a	Protein name	Accession no.b	Signal peptidec	pId		Masse		Gene location
				Exptl	Theoretical	Exptl	Theoretical
Proteins identified directly by MS in RA3:00
36	Sap	NP_654829	+	6.29	6.89	100.3	86.7	Chrf
24	Sap	NP_654829	+	6.47	6.89	101.1	86.7	Chr
38	Sap	NP_654829	+	6.71	6.89	85.6	86.7	Chr
3	Sap	NP_654829	+	6.42	6.89	84.3	86.7	Chr
39	Phosphoglycerate mutase	NP_834804	−	5.04	4.86	67.6	56.9	Chr
40	Phosphoglycerate mutase	NP_981532	−	4.90	4.84	64.0	53.4	Chr
37	Oligopeptide ABC transporter	NP_843667	+	6.00	6.34	60.4	61.7	Chr
5	Oligopeptide ABC transporter	NP_843667	+	6.41	6.56	63.2	61.8	Chr
41	EA1	NP_654830	+	5.90	5.70	58.4	91.4	Chr
42	Isocitrate Lyase	NP_830914	−	6.08	5.27	53.8	47.1	Chr
43	Enolase	NP_653583	−	4.73	4.66	53.3	46.6	Chr
11	Enolase	NP_653583	−	4.74	4.66	50.6	46.6	Chr
44	Enolase	NP_653583	−	4.77	4.66	53.3	46.6	Chr
28	Alanine dehydrogenase	NP_658654	−	5.37	5.22	42.6	40.3	Chr
45	Fructose-1,6-bisphosphatase	NP_847733	−	5.79	5.53	39.7	34.2	Chr
31	Malate dehydrogenase	NP_658620	+	5.39	5.12	42.0	33.6	Chr
46	S-layer homology domain	NP_657198	+	5.40	5.84	41.9	40.6	Chr
47	EA1	NP_654830	+	6.01	5.70	32.9	91.4	Chr
48	Bacillolysin	NP_654541	+	5.83	5.82	39.5	61.0	Chr
49	Alkyl hydroperoxide reductase	NP_654276	−	4.68	4.81	23.6	20.9	Chr
Proteins identified by positional overlapg
1	Sap	NP_654829	+	5.73	6.89	103.9	86.7	Chr
2	EA1	NP_654830	+	5.81	5.70	102.5	91.4	Chr
23	EA1	NP_654830	+	6.10	5.70	101.6	91.4	Chr
24	Sap	NP_654829	+	6.44	6.89	98.6	86.7	Chr
4	Oligopeptide ABC transporter	NP_843667	+	6.20	6.56	62.9	61.8	Chr
6	Oligopeptide ABC transporter	NP_843667	+	6.25	6.56	60.8	61.8	Chr
11	Enolase	NP_653583	−	4.76	4.66	51.8	46.6	Chr
12	Enolase	NP_653583	−	4.82	4.66	51.9	46.6	Chr
26	Phosphoglycerol transferase	NP_834895	−	5.20	5.37	48.0	44.9	Chr
27	Cell surface protein A	NP_658575	+	5.13	5.56	48.1	99.9	Chr
29	Glyceraldehyde 3-phosphate dehydrogenase	NP_834805	−	5.73	5.04	44.1	35.5	Chr
13	S-layer homology domain	NP_657198	+	5.31	5.84	41.8	40.6	Chr
17	Triosephosphate isomerase	NP_653585	−	5.17	5.00	29.5	26.7	Chr
22	Putative nucleoside diphosphate kinase	NP_655416	−	5.53	5.24	15.3	16.7	Chr

^aSpot numbers refer to Fig. 2B.

^bAccession numbers available through National Center for Biotechnology Information.

^cPresence of signal peptide predicted by SignalP 3.0 (+, yes; −, no).

^dIsoelectric point. Exptl, experimental.

^eMolecular mass (kDa). Exptl, experimental.

^fChr, chromosome.

^gOf proteins identified from RA3 and RA3R by MS.